Plevako put a spiral of silver wire, containing 99.999% silver, 20 m and a diameter of 0.4 mm into the vessel with 100 ml of distilled water. After 24, 48 and 72 h bactericidal activity tested the water for bacteria coli. The contact time with bacteria was 24 h and then was placed on conventional culturing medium.

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The wire was tested with both washed (25% solution of ammonia), and washed with at the surface. Experiments proved that the laundered money loses its bactericidal properties. Similar experiments carried out with silver sand, confirmed the view set out above. It is equally illustrative of the results achieved, researchers Thiele and Wolf. They experimentally studied the bactericidal effect of several galvanic couples: a silver sheet - gold, silver - platinum, silver powder - palladium, charcoal - silver. Thiele and Wolf showed that galvanic cells formed are soluble biologically active electrodes.

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Around the cathode of the galvanic couples sterile zone was not observed at all or was insignificant, while the round silver plate was wider than the sterile zone around the disc of the same metal that is not included in the electrode pair. Adding 0.00005 grams of coal to 1 g of silver powder significantly increased the bactericidal effect. Research the author of this booklet, as well as the latest published work of other researchers to confirm the above facts and suggest that it is metal ions and ionic compounds (substances that can dissolve in water into ions) cause the death of microorganisms. In all cases, the bactericidal effect of the degree of activity of silver is greater, the higher the concentration of ions in solution.

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The formation of the electrode pairs contributes to the transition metal in the active solution in the form of ions. For the same reason, the oxidized metal (coated with a film consisting of oxides or peroxides of the same metal) have greater activity than neoksidirovannye. Foreign matter in the water in a negative impact if they bind silver ions in a little dissociated or sparingly soluble compounds precipitated.